Part:BBa_K317040
Pconst.(L)-RBS-LuxI-Double term-Plac-Lysis
This devicce includes BBa_K112808, the lysis device using three genes from enterobacteria phage T4: lysozyme, holin, and antiholin.
Evaluation of e BBa_K317040 for the characterization and application of BBa_K112808
We performed a experiment with this device in order to characterize BBa_K112808 activity.The experiment was performed from single colonies in a streaked LB agar plate.At first, we cultured the single colony having the above device in 100 mL LB for 12 hors at 37℃ with agitation.Then, the culture was dispensed into 3 mL scale and IPTG(f.c. 0.3 mM) was added to each dispensed culture to induce the expression of Lysis genes. The obtained culture was incubated for 20 hours at room temperature without agitation.After that, the OD660 of each culture(N=3) was measured.The result was shown below(Fig).
The result showed that the OD660 were different in presence of IPTG ( orange bar) or not (gray bar).
OD660 of samples with the IPTG were half less than no additive samples. That indicates IPTG induced the expression of lysis genes and the E.coli in the culture were lysed.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 2850
Illegal NheI site found at 2873 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2455
Illegal AgeI site found at 2525 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 3106
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